Perbandingan Pengukuran Kadar Vitamin C Menggunakan Spektrofotometri UV-Vis pada Panjang Gelombang UV dan Visible

Comparison of Measurement The Vitamin C Level using UV-Vis Spetrophotometry at Uv and Visible Wavelength

Keywords: vitamin C, UV-Vis spectrophotometry, UV wavelength, Visible wavelength

Abstract

Vitamin C is a source of antioxidants which has a primary function to inhibit the occurrence of an oxidation reaction and inhibit a free radical. The source of vitamin C can be found in food and beverage. This study aims to compare the measurement of vitamin C levels using a UV-Vis spectrophotometer at UV and Visible wavelength. The method used in this study was the UV-Vis spectrophotometry method. The analysis of vitamin C levels at UV wavelength 266 nm and Visible wavelength 494 nm with a calibration curve range 0.3 to 0.8 mg/L. Validation of the methods studied included: linearity, detection limits, quantization limits, and precision. The results showed that the measurement of vitamin C levels at UV wavelengths resulted in the linearity of concentrations of 0.2 - 0.8 mg / L, the limit of detection of 0.05 mg/L, the limit of quantization of 0.17 mg/L and level of precision with RSD to 0.2015. On the other hand, the measurement of vitamin C levels in the Visible wavelength produced linearity from concentrations of 0.3 to 0.8 mg/L, the detection limit of 0.05 mg/L, quantization limit of 0.18 mg/L and the level of precision with RSD of 1.0489. Thus, the determination of vitamin C levels in samples using a UV-Vis spectrophotometer was preferred to be measured at UV wavelengths.

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References

Arny, N., Putri, A. N., Andina, L. (n.d.). Analisis Kuantitatif Vitamin C Dalam Injeksi Whitening Menggunakan Metode Spektrofotometri Uv-Vis.
Buhari, I. 2010. Analisis Kadar Vitamin C dalam Produk Olahan Buah Salak (Salacca zalacca) secara Spektrofotometri UV-Vis. Universitas Islam Negeri Alauddin Makassar.
Harmita, H. 2012. Petunjuk pelaksanaan validasi smetode dan Cara Perhitungannya. Pharmaceutical Sciences and Research (PSR), 1(3): 117–135.
Karinda, M., Fatimawali, F., Citraningtyas, G. 2013. Perbandingan Hasil Penetapan Kadar Vitamin C Mangga Dodol dengan Menggunakan Metode Spektrofotometri UV-Vis dan Iodometri. PHARMACON, 2(1): 86–89.
Lestari, P., Sabikis, S.,Utami, P. I. 2016. Analisis Natrium Nitrit Secara Spektrofotometri Visibel Dalam Daging Burger Yang Beredar Di Swalayan Purwokerto. PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia), 8(3).
Mulyani, E. 2018. Perbandingan Hasil Penetapan Kadar Vitamin C pada Buah Kiwi (Actinidia deliciousa) dengan Menggunakan Metode Iodimetri dan Spektrofotometri UV-Vis. Pharmauho, 3(2).
Pratama, M.R.F., Suratno, S., & Mulyani, E. 2018. Profile of Thin-Layer Chromatography and UV-Vis Spectrophotometry of Akar Kuning Stem Extract (Arcangelisia flava). Borneo Journal of Pharmacy. 1(2):72-76.
Rahmawati, F., & Hana, C. 2016. PENETAPAN Kadar Vitamin C Pada Bawang Putih (Allium Sativum, L) Dengan Metode Iodimetri. Cerata Jurnal Ilmu Farmasi (Journal of Pharmacy Science), 4(1).
Romsiah, R., Marista, S. L., & Fatoni, A. 2017. Validasi Metode Dan Penetapan Kadar Nitrit (No2-) Pada Sosis Sapi Curah Dan Sosis Sapi Kaleng Yang Dijual Di Swalayan Kota Palembang Secara Spektrofotometri Uv-Vis. Scientia-Journal of Pharmacy and Health, 7(2), 113–119.
Siti, N., Agustina, A., Nurhaini, R. 2016. Penetapan Kadar Vitamin C Pada Jerami Nangka (Artocarpus Heterpophyllus L.). Jurnal Farmasi Sains Dan Praktis, 2(1): 1–5.
Tahir, M., Kusuma, A. T., Ekawati, E. 2018. Analysis of Lycopene and Vitamin C Levels of Pomelo Citrus Fruit (Citrus maxima (Burm) Merr) Red n White Varieties From South Sulawesi. JCPS (Journal of Current Pharmaceutical Sciences), 2(1): 125–130.
Published
2019-04-30
How to Cite
Ngibad, K., & Herawati, D. (2019). Perbandingan Pengukuran Kadar Vitamin C Menggunakan Spektrofotometri UV-Vis pada Panjang Gelombang UV dan Visible. Borneo Journal of Medical Laboratory Technology, 1(2), 77-81. https://doi.org/10.33084/bjmlt.v1i2.715