TY - JOUR AU - Ngibad, Khoirul AU - Herawati, Dheasy PY - 2019/04/30 Y2 - 2024/03/29 TI - Perbandingan Pengukuran Kadar Vitamin C Menggunakan Spektrofotometri UV-Vis pada Panjang Gelombang UV dan Visible: Comparison of Measurement The Vitamin C Level using UV-Vis Spetrophotometry at Uv and Visible Wavelength JF - Borneo Journal of Medical Laboratory Technology JA - Borneo J Med Lab Tech VL - 1 IS - 2 SE - Articles DO - 10.33084/bjmlt.v1i2.715 UR - https://journal.umpr.ac.id/index.php/bjmlt/article/view/715 SP - 77-81 AB - <p style="text-align: justify;">Vitamin C is a source of antioxidants which has a primary function to inhibit the occurrence of an oxidation reaction and inhibit a free radical. The source of vitamin C can be found in food and beverage. This study aims to compare the measurement of vitamin C levels using a UV-Vis spectrophotometer at UV and Visible wavelength. The method used in this study was the UV-Vis spectrophotometry method. The analysis of vitamin C levels at UV wavelength 266 nm and Visible wavelength 494 nm with a calibration curve range 0.3 to 0.8 mg/L. Validation of the methods studied included: linearity, detection limits, quantization limits, and precision. The results showed that the measurement of vitamin C levels at UV wavelengths resulted in the linearity of concentrations of 0.2 - 0.8 mg / L, the limit of detection of 0.05 mg/L, the limit of quantization of 0.17 mg/L and level of precision with RSD to 0.2015. On the other hand, the measurement of vitamin C levels in the Visible wavelength produced linearity from concentrations of 0.3 to 0.8 mg/L, the detection limit of 0.05 mg/L, quantization limit of 0.18 mg/L and the level of precision with RSD of 1.0489. Thus, the determination of vitamin C levels in samples using a UV-Vis spectrophotometer was preferred to be measured at UV wavelengths.</p> ER -