Profile of Thin-Layer Chromatography and UV-Vis Spectrophotometry of Akar Kuning Stem Extract (Arcangelisia flava)
This study aims to obtain the profile of Thin-Layer Chromatography (TLC) and Ultraviolet-Visible (UV-Vis) spectrophotometry from the ethanol extract of akar kuning stems (Arcangelisia flava) from Central Kalimantan. The TLC method is used with the orientation phase of the combination of polar-non-polar solvents resulting from orientation, while ethanol is used as the solvent for UV-Vis spectrophotometers. TLC results showed the formation of three stains on a mixture of polar solvents chloroform : methanol : water while in a non-polar solvent combination n-hexane : ethyl acetate did not show any stains. Comparison of retention factor (Rf) values shows the best combination of polar solvents to separate stains at a ratio of 5 : 2 : 1, respectively. Separation in 2-dimensional TLC with polar solvents showed a similar pattern with 1-dimensional separation in the form of three stains. UV-Vis spectrophotometer results showed four main peaks with wavelength 227.2; 267.4; 345.2; and 425.3 nm, respectively. The profile of the peak formed is very similar to that shown by berberine, one of the primary metabolites of akar kuning. TLC and UV-Vis spectrophotometers profiles obtained are expected to support further research using akar kuning stems, especially those from Central Kalimantan.
Azwanida, N.N. 2015. A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation. Medicinal & Aromatic Plants. 4(3):1000196.
Behera, S., Ghanty, S., Ahmad, F., Santra, S., Banerjee, S. 2012. UV-Visible Spectrophotometric Method Development and Validation of Assay of Paracetamol Tablet Formulation. Journal of Analytical & Bioanalytical Techniques. 3(6):1000151.
Bele, A.A., Khale, A. 2011. An overview on thin layer chromatography. International Journal of Pharmaceutical Sciences and Research. 2(2):256-267.
Coskun, O. 2016. Separation techniques: Chromatography. Northern Clinics of Istanbul. 3(2):156-160.
Dai, J., Mumper, R.J. 2010. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules. 15(10):7313-7352.
Desmukh, S.A., Gaikwad, D.K. 2014. A review of the taxonomy, ethnobotany, phytochemistry and pharmacology of Basella alba (Basellaceae). Journal of Applied Pharmaceutical Science. 4(1):153-165.
Fahrianoor, Windari, T., Taharuddin, Ruslimar’I, Maryono. 2013. The Practice of Local Wisdom of Dayak People in Forest Conservation in South Kalimantan. Journal of Wetlands Environment Management. 1(1):33-41.
Guerriero, G., Berni, R., Munoz-Sanchez, J.A., Apone, F., Abdel-Salam, E.M., Qahtan, A.A., Alatar, A.A., Cantini, C., Cai, G., Hausman, J.F., Siddiqui, K.S., Hernandez-Sotomayor, S.M., Faisal, M. 2018. Review: Production of plant secondary metabolites: examples, tips and suggestions for biotechnologists. Genes. 9(6):pii:E309.
Haug, M. 2014. Resistance, Ritual Purification and Mediation: Tracing a Dayak Community's Sixteen-Year Search for Justice in East Kalimantan. The Asia Pacific Journal of Anthropology. 15(4):357-375.
Kagan, I.A., Flythe, M.D. 2014. Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds. Journal of Visualized Experiments. (85):51411.
Kaijanen, L., Paakkunainen, M., Pietarinen, S., Jernstrom, E., Reinikainen, S.P. 2015. Ultraviolet Detection of Monosaccharides: Multiple Wavelength Strategy to Evaluate Results after Capillary Zone Electrophoretic Separation. International Journal of Electrochemical Science. 10:2950-2961.
Koide, T., Iwata, M., Maekawa, K., Saito, H., Tanimoto, T., Okada, S. 2001. [Berberine hydrochloride reference standard (Control 001) of National Institute of Health Sciences]. Kokuritsu Iyakuhin Shokuhin Eiken Kenkyusho Hokoku. 119:97-100.
Kolb, C.A., Kaser, M.A., Kopecky, J., Zotz, G., Riederer, M., Pfundel, E.E. 2001. Effects of natural intensities of visible and ultraviolet radiation on epidermal ultraviolet screening and photosynthesis in grape leaves. Plant Physiology. 127(3):863-875.
Maryani, Marsoedi, Nursyam, H., & Maftuch. 2013. The Phytochemistry and The Anti-Bacterial Activity of Yellow Root (Arcangelisia flava Merr) against Aeromonas hydrophila. Journal of Biology and Life Science. 4(2):180-190.
Matsyik, E., Wozniak, A., Paduch, R., Rejdak, R., Polak, B., Donica, H. 2016. The new TLC method for separation and determination of multicomponent mixtures of plant extracts. Journal of Analytical Methods in Chemistry. 1813581.
Pratama, M.R.F. 2016. Akar kuning (Arcangelisia flava) sebagai inhibitor EGFR: Kajian in silico. Jurnal Farmagazine. 3(1):6-16.
Pratama, M.R.F. 2017. Akar Kuning (Arcangelisia flava) as Neuraminidase Inhibitor: Molecular Docking and Pharmacophore Optimization Approach. Advances in Health Sciences Research. 6:502-511.
Pratama, M.R.F., Pratomo, G.S. 2017. Pharmacophore optimization of berberine as HER2 inhibitor. Jurnal Farmasi Indonesia. 14(2):109-117.
Pundarikakshudu, K., Dave, H.N. 2010. Simultaneous determination of curcumin and berberine in their pure form and from the combined extracts of Curcuma longa and Berberis aristata. International Journal of Applied Science and Engineering. 8(1):19-26.
Setyowati, F.M. 2010. Etnofarmakologi dan Pemakaian Tanaman Obat Suku Dayak Tunjung di Kalimantan Timur. Media Litbang Kesehatan. 20(3):104-112.
Stoll, D.R., Li, X., Wang, X., Carr, P.W., Porter, S.E.G., Rutan, S.C. 2011. Fast, comprehensive two dimensional liquid chromatography. Journal of Chromatography A. 1168(1-2):3-2.
Subiandono, E. & Heriyanto, N.M. 2009. Kajian Tumbuhan Obat Akar Kuning (Arcangelisia flava Merr.) di Kelompok Hutan Gelawan, Kabupaten Kampar, Riau. Buletin Plasma Nutfah. 15(1):43-48.
Wahyuono, S., Setiadi, J., Santosa, D., Hartati, M.S., Soekotjo, Muslimah, S., Prihatiningtias, W. 2006. Identifikasi Struktur Senyawa Bioaktif dari Akar Kuning (Fibraurea chloroleuca Miers.) (#03-SBK-029) Koleksi dari Hutan Kalimantan Tengah. Majalah Obat Tradisional. 12(39):1-8.
Wang, H., Zhu, C., Ying, Y., Luo, L., Huang, D., Luo, Z. 2018. Metformin and berberine, two versatile drugs in treatment of common metabolic diseases. Oncotarget. 9(11):10135-10146.
Widi, R.K., Indriati, T. 2007. Penjaringan dan Identifikasi Senyawa Alkaloid dalam Batang Kayu Kuning (Arcangelisia flava Merr). Jurnal Ilmu Dasar. 8(1):24-29.
Copyright (c) 2018 Mohammad Rizki Fadhil Pratama, Suratno Suratno, Evi Mulyani
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors continue to retain the copyright to the article if the article is published in the Borneo Journal of Pharmacy. They will also retain the publishing rights to the article without any restrictions.
Authors who publish with this journal agree to the following terms:
- Any article on the copyright is retained by the author(s).
- The author grants the journal, right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share work with an acknowledgement of the work authors and initial publications in this journal.
- Authors are able to enter into a separate, additional contractual arrangements for the non-exclusive distribution of published articles of work (eg, post-institutional repository) or publish it in a book, with acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their websites) prior to and during the submission process, as can lead to productive exchanges, as well as earlier and greater citation of published work.
- The article and any associated published material is distributed under the Creative Commons Attribution-ShareAlike 4.0 International License