Anti-inflammatory Activity of Pharmaceutical Gel of Ethanolic Extract from Marine Sponge Xestospongia Sp

  • Wa Ode Sitti Zubaydah Universitas Halu Oleo
  • Wahyuni Wahyuni Universitas Halu Oleo
  • Sahidin Sahidin Universitas Halu Oleo
  • Tian Amalia Halik Universitas Halu Oleo
  • Rina Andriani Universitas Halu Oleo
  • Astrid Indalifiany Universitas Halu Oleo
  • Adryan Fristiohady Universitas Halu Oleo http://orcid.org/0000-0002-3499-0511
Keywords: Gel, Ethanol extract, Xestospongia Sp. sponge, Carrageenan, Anti-inflammatory

Abstract

The aim of this study was to determine the characteristics of the gel formula based on it’s organoleptic properties, pH, viscosity, dispersion and homogenity by stability test carried out using the cycling test method and to determine the anti-inflammatory activity of the Xestospongia Sp. in male white mice (Mus musculus) by creating an artificial edema on the mice left foot induced by 1% λ-carrageenan. The gel formula from the ethanol extract of Xestospongia Sp. sponge was physically stable in terms of its organoleptic observation, homogenity, pH and viscosity test. However, the results that were obtained after dispersion test did not fulfill the requirements. In this study, the gel formula of the ethanol extract of Xestospongia Sp. sponge was administered on the mice left foot by using the variations in extract concentration of 0.02%, 0.03%, and 0.04%, and the gel without extract as a negative control and Galtaren®gel (1% Diclofenac Sodium) as a positive control. The evaluated data were in the form of mice leg edema volume measured based on its percent of inflammation and percent of inflammatory inhibition and observed for 360 minutes. The data were analyzed by using the Kruskal-Wallis test followed by the Mann-Whitney test with a confidence level of 95%. The results of this study showed that the gel formula of ethanol extract of Xestospongia Sp. sponge has an anti-inflammatory effect on each concentration and the formula that has a large anti-inflammatory effect was obtained at extract concentration of 0.04%.

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References

Amri, M.U. 2018. Uji Aktivitas Antiinflamasi dengan Metode Stabilisasi Membran Sel Darah Merah Secara In Vitro dan Uji Toksisitas Akut Terhadap Larva Udang Artemia Salina Leach Ekstrak Etanol Spons Xestospongia Sp. Skripsi. Faculty of Pharmacy, Department of Pharmacy, Universitas Halu Oleo, Kendari.
Aswal, A., Kalra, M., Rout, A. 2013. Preparation and Evaluation of Polyherbal Cosmetic Cream. Der Pharmacia Lettre. 5(1):83-88.
Cribb, R., Ford, M. 2009. Indonesia as an archipelago: managing islands, managing the seas. In Singapore-Indonesia beyond the water's edge: managing an archipelagic state. Singapore: ISEAS-Yusof Ishak Institute.
Duarte, D.B., Vasko, M.R., Fehrenbacher, J.C. 2012. Models of Inflammation: Carrageenan Air Pouch. Current Protocols in Pharmacology. CHAPTER 5: Unit5.6.
El-Shitany, N.A., Shaala, L.A., Abbas, A.T., Abdel-Dayem, U.A., Azhar, E.I., Ali, S.S., van Soest, R.W., Youssef, D.T. 2015. Evaluation of the Anti-Inflammatory, Antioxidant and Immunomodulatory Effects of the Organic Extract of the Red Sea Marine Sponge Xestospongia testudinaria against Carrageenan Induced Rat Paw Inflammation. PLoS One. 10(9): e0138917.
Garg, A., Aggarwal, D, Garg, S., Singla, A.K. 2002. Spreading of Semisolid Formulation: An Update. Pharmaceutical Technology North America. 26(9):84-105.
Hardman, J.G., Limbird, L.E. 2003. Goodman & Gilman: Dasar Farmakologi Terapi. Ed.10. Vol.2. Jakarta: Penerbit Buku Kedokteran EGC.
Kosala, K., Widodo, M.A., Santoso, S., Karyono, S. 2018. In vitro and In vivo Anti-inflammatory Activities of Coptosapelta flavescens Korth Root’s Methanol Extract. Journal of Applied Pharmaceutical Science. 8(9):42-48.
Luliana, S., Susanti, R., Agustina, E. 2017. Uji Aktivitas Antiinflamasi Ekstrak Air Herba Ciplukan (Physalis angulata L.) terhadap Tikus Putih (Rattus norvegicus L.) Jantan Galur Wistar yang Diinduksi Karagenan. Majalah Obat Tradisional. 22(3):199-205.
Manurung, N.R.M., Sumiwi, S.A. 2016. Aktivitas Antiinflamasi Berbagai Tanaman Diduga Berasal dari Flavonoid. Farmaka Online. 14(2):111-123.
Mappa, T., Edy, H.J., Kojong, N. 2013. Formulasi Gel Ekstrak Daun Sasaladahan (Peperomia pellucida (L.) H.B.K) dan Uji Efektivitasnya Terhadap Luka Bakar pada Kelinci (Oryctolagus Cuniculus). Pharmacon. 2(2):49-55.
Marzuki, I. 2018. Eksplorasi Spons Indonesia: Seputar Kepulauan Spermonde. Makassar: Nas Media Pustaka.
Mehbub, M.F., Lei, J., Franco, C., Zhang, W. 2014. Marine Sponge Derived Natural Products between 2001 and 2010: Trends and Opportunities for Discovery of Bioactives. Marine Drugs. 12(8):4539-4577.
Ministry of Health of the Republic of Indonesia. 1995. Materia Medika Indonesia Edisi VI. Jakarta: Dirjen Pengawas Obat dan Makanan.
Murniasih, T. 2003. Metabolit Sekunder dari Spons Sebagai Bahan Obat-Obatan. Oseana. 28(3):27-33.
Pakki, E., Sartini, Tayed, R., Maisarah, N.L. 2009. Formulasi dan Evaluasi Kestabilan Fisik Gel Antioksidan Ekstrak Biji Kakao (Theobroma cacao L.). Majalah Farmasi dan Farmakologi. 13(2):1-7.
Rowe, R.C., Sheskey, P.J., Quinn, M.E. 2009. Handbook of Pharmaceutical Excipients 6th. London: American Pharmaceutical Association.
Sadarun, B., Malaka, M.H., Wahyuni, Sahidin. 2018. Senyawa Steroid Spons Xestospongia sp. dari Perairan Sulawesi Tenggara, Pharmauho: Jurnal Farmasi, Sains dan Kesehatan. 4(1):26-29.
Saraung, V., Yamlean, P.V., Citraningtyas, G. 2018, Pengaruh Variasi Basis Karbopol Dan HPMC pada Formulasi Gel Ekstrak Etanol Daun Tapak Kuda (Ipomoea Pes-Caprae (L.) R. Br. dan Uji Aktivitas Antibakteri Terhadap Staphylococcus Aureus. Pharmacon. 7(3):220-229.
Sativa, O., Yuliet, Y., Sulastri, E. 2014. Uji Aktivitas Antiinflamasi Gel Ekstrak Kaktus (Opuntia elatior Mill.) pada Tikus (Rattus norvegicus L.) yang Diinduksi Lamda Karagenan. Natural Science: Journal of Science and Technology. 3(2):79-94.
Sayuti, N.A. 2015. Formulasi dan Uji Stabilitas Fisik Sediaan Gel Ekstrak Daun Ketepeng Cina (Cassia alata L.). Jurnal Kefarmasian Indonesia. 5(2):74-82.
Sharon, N., Anam, S., Yuliet, Y. 2013, Formulasi Gel Antioksidan Ekstrak Etanol Bawang Hutan (Eleutherine palmifolia L. Merr). Natural Science: Journal of Science and Technology. 2(3):111-122.
Sujono, T.A., Patimah, R., Yuliani, R. 2012. Efek Antiinflamasi Infusa Rimpang Temu Putih (Curcuma zedoaria (Berg) Roscoe) pada Tikus yang Diinduksi Karagenin. Biomedika. 4(2):10-17.
Sukmawati, S., Yuliet, Y., Hardani, R. 2015. Uji Aktivitas Antiinflamasi Ekstrak Etanol Daun Pisang Ambon (Musa Paradisiaca L.) Terhadap Tikus Putih (Rattus Norvegicus L.) yang Diinduksi Karagenan. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy). 1(2):126-132.
Wathoni, N., Rusdiana, T., Hutagaol R.Y. 2009. Formulasi Gel Antioksidan Ekstrak Rimpang Lengkuas (Alpinia galanga L. Wild) dengan Menggunakan Basis Aqupec 505 HV. Farmaka Online. 7(1):15-27.
Widiyantoro, A., Destiarti, L., Kusharyanti, I., Supardi., Halim, D.G., Niwick, Willianti, V. 2012. Aktivitas Antiinflamasi Senyawa Bioaktif dari Kulit Batang Pauh Kijang (Irvingia malayana Oliv. ex. A. Benn) terhadap Tikus Putih (Rattus norvegicus) yang Diinduksi Karagenan. Kaunia: Jurnal Sains, Pendidikan Sains dan Teknologi. 8(2):118-126.
Wijaya, L., Saleh, I., Theodorus, T., Salni, S. 2015. Efek Antiinflamasi Fraksi Daun Andong (Cordyline Fruticosa L) Pada Tikus Putih Jantan (Rattus norvegicus) Galur Spraque Dawley. Biomedical Journal of Indonesia. 1(1):16-24.
Published
2019-05-04
How to Cite
Zubaydah, W. O. S., Wahyuni, W., Sahidin, S., Halik, T. A., Andriani, R., Indalifiany, A., & Fristiohady, A. (2019). Anti-inflammatory Activity of Pharmaceutical Gel of Ethanolic Extract from Marine Sponge Xestospongia Sp. Borneo Journal of Pharmacy, 2(1), 1-9. https://doi.org/10.33084/bjop.v2i1.694
Section
Pharmacology-Toxicology