Immunomodulator Activity of Effervescent Granule of Wualae Fruit (Etlingera elatior (Jack) R.M. Smith) Based on Specific Phagocytic Activity

  • Adryan Fristiohady Universitas Halu Oleo http://orcid.org/0000-0002-3499-0511
  • Wa Ode Siti Zubaydah Universitas Halu Oleo
  • Wahyuni Wahyuni Universitas Halu Oleo
  • Mirda Mirda Universitas Halu Oleo
  • Saripuddin Saripuddin Universitas Halu Oleo
  • Rina Andriani Universitas Halu Oleo
  • La Ode Muhammad Julian Purnama Universitas Halu Oleo
  • Sahidin Sahidin Universitas Halu Oleo https://orcid.org/0000-0003-0997-0065
Keywords: Effervescent granule, Wualae fruit, phagocytosis activity

Abstract

The previous study reported that Etlingera elatior (Wualae) has activity as immunostimulant with dose at 300 mg/kg BW and 400 mg/kg BW. Formulating natural product into effervescent granule (GE) can increase practicality and interest to consume traditional medicine. This study aims to investigate the immunostimulant activity of Wualae ethanolic extract in the effervescent granule. Wualae was macerated with ethanol then formulated into effervescent granule at dose 300 mg/kgBW and 400 mg/kgBW. Formulations were evaluated. Animals were divided into 4 groups and treated with: group I (positive control); group II (negative control); group III (GE dose at 300mg/kgBW); and group IV (GE at dose 400mg/kgBW) for 7 days and at days 8, groups were infected by Staphylococcus aureus intraperitoneally. Immunostimulant activity was measured by calculating the Specific Phagocytic Activity (SPA) of macrophage. Data collected from the evaluation and measurement of the SPA then analyzed using SPSS. Results from the evaluation were considered good except the moisture content of effervescent granule. Results from SPA was found that effervescent granule at dose 300 mg/kgBW and at dose 400 mg/kgBW have 26.33% and 32.5% consecutively with significance 0.02 (p<0.05). Thus, effervescent granule has comparable phagocytosis activity with positive control.

Downloads

Download data is not yet available.

References

Anwar, K. 2010. Formulasi Sediaan Tablet Effervescent dari Ekstrak Kunyit (Curcuma domestica Val.) dengan Variasi Jumlah Asam Sitrat-Asam Tartrat sebagai Sumber Asam. Jurnal Ilmiah Berkala Sains dan Terapan Kimia. 4(2):168-178. http://dx.doi.org/10.20527/jstk.v4i2.2065
British Pharmacopoeia Commission. 2009. British Pharmacopoeia 2009. London: The Stationery Office.
Chan, E.W.C., Lim, Y.Y., Wong, S.K. 2011. Phytochemistry and Pharmacological Properties of Etlingera elatior: A Review. Pharmacognosy Journal. 3(22):6-10. https://doi.org/10.5530/pj.2011.22.2
Duque, G.A., Descoteaux, A. 2014. Macrophage Cytokines: Involvement in Immunity and Infectious Diseases. Frontiers in Immunology. 5:491. https://dx.doi.org/10.3389/fimmu.2014.00491
Hosseinzade, A., Sagedhi, O., Biregani, A.N., Soukhtehzari, A., Brandt, G.S., Esmailzadeh, A. 2019. Immunomodulatory Effects of Flavonoids: Possible Induction of T CD4+ Regulatory Cells Through Suppression of mTOR Pathway Signaling Activity. Frontiers in Immunology. 10:51. https://dx.doi.org/10.3389/fimmu.2019.00051
Jaafar, F.M., Osman, C.P., Ismail, N.H., Awang, K. 2007. Analysis of Essential Oils of Leaves, Stems, Flowers and Rhizomes of Etlingera elatior (Jack) R. M. Smith. Malaysian Journal of Analytical Sciences. 11(1):269-273.
Lachumy, S.J.T., Sasidharan, S., Sumathy, V., Zuraini, Z. 2010. Pharmacological activity, phytochemical analysis and toxicity of methanol extract of Etlingera elatior (torch ginger) flowers. Asian Pacific Journal of Tropical Medicine. 3(10):769-774. https://doi.org/10.1016/S1995-7645(10)60185-X
Liu, Z., Sun, X., Liang, T., Luo, Y., Chen, X., Wang, J., Lin, Y., Ye, Y., Zhong, Z. 2019. Preparation and Characterization of the Biological Compound Effervescent Granule of Calcium Acetate. Current Pharmaceutical Biotechnology. 20(11). https://doi.org/10.2174/1389201020666190628144637
Marusin, S., Chairul, C. 2012. Efek ekstrak air dan alkohol pada siwak (Salvadora persica L.) terhadap peningkatan aktivitas dan kapasitas fagositosis sel makrofag. Media Penelitian dan Pengembangan Kesehatan. 22(1):38-44.
Murphy, K.M. 2012. Janeway's Immunobiology. 8th edition. New York: Garland Science.
Patel, S.G., Siddaiah, M. 2018. Formulation and evaluation of effervescent tablets: a review. Journal of Drug Delivery and Therapeutics. 8(6):296-303. https://doi.org/10.22270/jddt.v8i6.2021
Perez-Cano, F.J., Castell, M. 2016. Flavonoids, Inflammation and Immune System. Nutrients. 8(10):659. https://dx.doi.org/10.3390/nu8100659
Petricevich, V.L., Reynaud, E., Cruz, A.H., Possani, L.D. 2008. Macrophage activation, phagocytosis and intracellular calcium oscillations induced by scorpion toxins from Tityus serrulatus. Clinical and Experimental Immunology. 154(3):415-423. https://doi.org/10.1111/j.1365-2249.2008.03754.x
van Riet-Nales, D.A., Schobben, A.F., Vromans, H., Egberts, T.C., Rademaker, C.M. 2016. Safe and effective pharmacotherapy in infants and preschool children: importance of formulation aspects. Archives of Disease in Childhood. 101(7):662-669. https://doi.org/10.1136/archdischild-2015-308227
Swarbick, J. 2013. Encyclopedia of Pharmaceutical Science and Technology, Six Volume Set. 4th edition. Boca Raton: CRC Press.
Wahyuni, Malaka, M.H., Fristiohady, A., Yusuf, M.I., Sahidin. 2017. Potensi Imunomodulator Ekstrak Etanol Buah Kecombrang (Etlingera elatior (Jack) R.M.Smith) terhadap Aktivitas Fagositosis Makrofag Mencit Jantan Galur Balb/C. Pharmacon. 6(3):350-355.
Zalizar, L. 2013. Flavonoids of Phyllanthus Niruri as Immunomodulators a Prospect to Animal Disease Control. ARPN Journal of Science and Technology. 3(5):529-532.
Published
2019-11-14
How to Cite
Fristiohady, A., Zubaydah, W. O. S., Wahyuni, W., Mirda, M., Saripuddin, S., Andriani, R., Purnama, L. O. M. J., & Sahidin, S. (2019). Immunomodulator Activity of Effervescent Granule of Wualae Fruit (Etlingera elatior (Jack) R.M. Smith) Based on Specific Phagocytic Activity. Borneo Journal of Pharmacy, 2(2), 35-40. https://doi.org/10.33084/bjop.v2i2.868
Section
Pharmacology-Toxicology